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Abstract
[Full-Text
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Macedonian Journal of Medical Sciences. 2010 Jun 15;
3(2):132-137.
doi:10.3889/MJMS.1857-5773.2010.0101
Basic Science
Antioxidant Effects of Nigella Sativa in the Treatment of Experimentally Induced Rhinosinusitis
Ozgur Yoruk1,
Fatma Ozabacigil Gur2, Hamidullah Uyanik3, Murat Yasar1, Vahit Mutlu1, Enver
Altas1, Elif Baysal4, Seyithan Taysi5
1Department of
Otorhinolaryngology-Head and Neck Surgery, School of Medicine, Ataturk
University, Erzurum, Turkey; 2Department of Biochemistry, School of
Medicine, Ataturk University, Erzurum, Turkey; 3Department of Microbiology,
School of Medicine, Ataturk University, Erzurum, Turkey; 4Department of
Otorhinolaryngology-Head and Neck Surgery, School of Medicine, Gaziantep
University, Gaziantep, Turkey; 5Department of Biochemistry and Clinical
Biochemistry, School of Medicine, Gaziantep University, Gaziantep, Turkey.
Aim. The objective of this study was to investigate the effect of
Nigella sativa (NS) in experimental bacterial rhinosinusitis.
Material and Methods. Bacterial rhinosinusitis was induced with
Staphylococcus aureus. Rabbits were divided into control, NS 50,
NS 100 and NS 200 mg/kg/d groups. NS was given orally for
7 days. The same volume of normal saline was given as a vehicle to the
control group for the same period. At 7 days post-treatment, mucosal samples
were excised from the treated and control groups for measurements of
superoxide dismutase (SOD), glutathione peroxidase (GSH-Px), myeloperoxidase
(MPO) and malondialdehyde (MDA).
Results. SOD and GSH-Px activities significantly increased in the NS
50, NS 100 and NS 200 mg/kg/d groups when compared with the
saline treated group. MPO activity and MDA levels significantly decreased in
the NS 50, NS 100 and NS 200 mg/kg/d when compared with
the saline treated group.
Conclusion.
These findings show that administration of NS increased the SOD, GSH-Px
activities and decreased the lipid peroxidation and MPO activity in
experimental rhinosinusitis in rabbits. NS prevented oxidative stress
by scavenging reactive oxygen species generated in rhinosinusitis model in
rabbits.
Key words: Superoxide dismutase; experimental rhinosinusitis; lipid peroxidation; free radical; myeloperoxidase.
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