Elena Trajkovska-Dokic1, Snezana Stojkovska2, Kostadin
Icev1, Aleksandra Grozdanova3
1Institute of Microbiology and Parasitology, Medical Faculty,
“Ss Cyril and Methodius” University, Skopje, Republic of Macedonia; 2University
Clinic for Infective Diseases, Skopje, Republic of Macedonia; 3Faculty
of Pharmacy, “Ss Cyril and Methodius” University, Skopje, Republic of
Macedonia
Background: Thermophilic campylobacters are of worldwide significance
in human and animal diseases. Sources of human infection remain mainly
undetermined, but contaminated food, like poultry and row milk, are widely
regarded as important vehicles of infection. Accurate methods of strain
identification, differentiation and typing are essential for diagnosing and
epidemiological purposes.
Aim: The aim of our study was to determine the serologic and genetic
diversity among the strains and to assess the discriminatory power of both
typing methods in epidemiological studies.
Material and Methods: Within a period of six months were isolated 26
strains of C. jejuni from faecal samples of children with acute
gastroenteritis. Heat-stable specific antigen of C. jejuni was used
for serogrouping of the strains by the reaction of passive hemagglutination.
Purified genomic DNA was obtained and used in RAPD-PCR reaction.
Results: Twenty one C. jejuni strains belonged to the
following Penner’s serogroups: 8 strains were group A, 13 strains were group
O. The remaining 5 strains were non-typable. RAPD-PCR analysis of 26 strains
of Campylobacter jejuni yielded multiple amplification products in
all of them. None of the strains processed by this method was non-typable.
According to the number and sizes of amplification bands, 4 different
genotypes (a, b, c, d) of C. jejuni strains were distinguished within
26 investigated strains.
Conclusion: In this study we found that RAPD-PCR analysis provide
better discrimination of C. jejuni strains than serogrouping by
Penner’s method. Each of the three Penner’s antigenic groups comprised
different genotypes. RAPD-PCR analysis of C. jejuni resulted in the
generation of highly specific and reproducible DNA fingerprints that enable
discrimination even between isolates of a single bacterial serogroup.
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Citation: Trajkovska-Dokic E, Stojkovska S, Icev K, Grozdanova A.
Serogrouping and Randomly Amplified Polymorphic DNA Fingerprinting of
Campylobacter Jejuni. Maced J Med Sci. 2011 Dec 15; 4(4):372-375.
http://dx.doi.org/10.3889/MJMS.1957-5773.2011.0200.
Key words: Campylobacter jejuni; serogrouping; RAPD-PCR
fingerprinting.
Correspondence: Elena Trajkovska-Dokic, MD, PhD. Institute of
Microbiology and Parasitology, Medical Faculty, Skopje, Republic of
Macedonia. E-mail: elenatdokic@gmail.com
Received: 21-Oct-2011; Accepted: 13-Nov-2011; Online first: 25-Nov-2011
Copyright: © 2011 Trajkovska-Dokic E. This is an open access article
distributed under the terms of the Creative Commons Attribution License,
which permits unrestricted use, distribution, and reproduction in any
medium, provided the original author and source are credited.
Competing Interests: The authors have declared that no competing
interests exist.
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